European Journal of Nutrition (v.20, #1)

Keywords: arteriosclerogenesis; capillary basement membrane thickening; heart infarction; protein storage; risk factor diseases

Acid and alkaline phosphomonoesterases in Egyptian snake venoms by F. Hassan; Prof. Dr. M. F. S. El-Hawary; A. El-Ghazawy (44-54).
Non-specific acid and alkaline phosphomonoesterases could be demonstrated in two viperids (Cerastes cerastes andCerastes vipera) and two elapids (Naja haje andNaja nigricollis). The latter could be a natural source for the production of these enzymes.The activities of both enzymes in elapids were greater than in viperids.N. nigricollis was the only to show acid phosphatase activity exceeding its alkaline one.The optimum pH values recorded for acid phosphatase was 4.0 and 4.9 and for alkaline phosphatase 9.0 and 10.0 in viperids and elapids, respectively.Optimum substrate concentration for both enzymes in viperids was 0.01 M, while for acid phosphatase inN. haje andN. nigricollis it was 0.125 and 0.150 M; and for their alkaline phosphatases the values were 0.150 and 0.125 M, respectively.Mg++ behaved as an activator for both enzymes in all venoms investigated, while Zn++ showed either no or slight activating effect. Fluoride ions as well as EDTA showed certain inhibitory action.Both enzymes in the crude venoms were heat-labile.
Keywords: elapids; viperids; phosphomonoesterases

Durch thermische Denaturierung geht die ImmunogenitÄt von Sojaprotein verloren. Inkubation mit 6m-Guanidin-HCl stellt die ImmunogenitÄt wieder her. Durch dieses Reagens wird die bei Autoklavieren beobachtete Assoziation eines Teils der Sojaproteine wieder rückgÄngig gemacht. Dies dürfte die Hauptursache für die Rückgewinnung der ImmunogenitÄt sein. Diese Befunde werden im Zusammenhang mit der Bestimmung von autoklaviertem Sojaprotein durch zwei immunologische Verfahren diskutiert: Solid-Phase Radioimmunoassay und passive HÄmagglutination (Inhibitionsverfahren).Soy-bean protein loses its immunogenicity by heat treatment through thermal denaturation. Incubation with 6m guanidine-hydrochloride restores the immunogenicity. This reagent causes dissociation of protein aggregates formed during autoclaving of soy protein. This seems to be the major step in the restitution of immunogenicity. These findings are discussed in connection with the quantification of heat-denaturated soy-bean protein by two independent immunologic methods: Solid-Phase Radioimmunoassay and Passive Hemagglutination (inhibition test).
Keywords: Sojaprotein; Radioimmunoassay (Radioimmuntest); Proteindenaturierung; Passive HÄmagglutination (Sojamehl)

In-vivo studies on C2 organic acids in the tissues of rats injected with xylitol and glucose by S. Hauschildt; R. A. Chalmers; A. M. Lawson; K. Brand (69-75).
Oxalic, glyoxylic, and glycollic acid were determined in rat liver and kidney after injection with [U-14C]-xylitol or [U-14C]-glucose. Neither glucose nor xylitol led to the formation of oxalic and glyoxylic acid, yet glycollic acid was found in both tissues after injection with xylitol.Possible pathways leading from xylitol to glycollic acid are discussed.Oxal-, Glyoxyl- und GlykollsÄure wurden in Rattenleber und -niere nach Injektion von [U-14C]-Xylit oder [U-14C]-Glucose bestimmt. Weder Glucose noch Xylit führten zu einer Oxal- oder GlyoxylsÄurebildung. GlycollsÄure wurde jedoch in beiden Geweben nach Injektion von Xylit gefunden.Mögliche Stoffwechselwege vom Xylit zur GlykollsÄure werden diskutiert.

Buchbesprechungen by K. Lang; W. Seibel; H. Förster; Grüttner; H. Glatzel; D. Senczek; H. -D. Belitz (76-80).