European Journal of Nutrition (v.6, #2-3)

Kobalt- und Vitamin B12-Stoffwechsel by K. H. Menke; B. Marquering (77-83).
Chlortetracyclin (20–100 mg/kg) senkt in den ersten Stunden nach oraler Verabreichung von 50 mμMol60CoCl2 die Ausscheidung von Kobalt und Corrinoiden beim Huhn. Dieser Befund und die veränderten Kobalt- und Corrinoidgehalte in den Caeca lassen den Schluß zu, daß Chlortetracyclin die Zahl der Teilentleerungen des Caecums pro Zeiteinheit verringert. Infolgedessen steigt in den Caeca der Anteil vollständiger Purincobamide. Ein Einfluß auf die Bildung von Vitamin B12 konnte nicht nachgewiesen werden.5,6-Dimethylbenzimidazol (DMBIA) hingegen bewirkt als Hydrochlorid eine Steigerung der B12-Synthese in der Henne von weniger als 0,2μg auf 3μg je Tier und Tag, während in Äthanol gelöstes, feindispers eingemischtes DMBIA eine B12-Bildung von 2μg/Tag bewirkt und kristallines DMBIA keinen nennenswerten Effekt aufweist. DMBIA kann offenbar im Darmtrakt nicht synthetisiert werden und ist auch in den üblichen Futtermitteln nicht enthalten.Chlortetracycline decreases the excretion of cobalt and corrinoids in the first hours after oral administration of 50 mμMol60CoCl2. This effect and the altered cobalt- and corrinoid contents of the caeca lead to the conclusion, that chlortetracycline diminishes the number of partial depletions of the caecum per time. There is no influence on the formation of vitamin B12.5,6-dimethylbenzimidazol (DMBIA) fed as hydrochlorid to hens, causes an increase in B12-synthesis from 0,2 to 3μg per animal and day. DMBIA in ethanol solution increases B12-formation to 2μg per day and crystalline DMBIA has only a very small effect. This base certainly is not synthesized in the intestine and is usually not supplied by the feedstuff.

Alimentary production of gallstones in hamsters by H. Dam; I. Prange; F. Christensen (97-106).
Young hamsters were reared on diets containing butter fat and fat of a high linoleic acid margarine, respectively.The basal diet without the fat had previously been shown to produce a very high incidence of cholesterol gallstones in young hamsters.In two trials, in which the fats were compared at the 10% dietary level, the incidence of cholesterol galstones was much less with the diets containing margarine fat than with the diets containing butter fat. The difference was significant with a probability of more than 99%.In a third trial, in which the fats were compared at the 3% dietary level, the incidence of cholesterol gallstones was also less with margarine fat than with butter fat. Here the difference was significant with a probability of more than 95% but less than 99%.The superior effect of the margarine fat in counteracting the development of cholesterol gallstones is ascribed to its high content (about 40%) of linoleic acid.In one of the trials in which the two fats were given at the 10% dietary level the bladder bile was analyzed for cholesterol, bile acids and lipid soluble phosphorus, with the following results:For the animals receiving the butter fat the concentrations of cholesterol were found to be more scattered and, in more than half of the cases, higher than for animals receiving margarine fat.The ratios between the molar concentrations of bile acids and cholesterol and between the molar concentrations of lipid soluble phosphorus and cholesterol were also more scattered for animals receiving the butter fat diet, but in more than half of the cases in the butter fat group these ratios were lower than in the margarine fat group.In the butter fat group the values for the two aforementioned ratios tended to decrease with increasing concentrations of cholesterol.A more exact correlation of the composition of the bladder bile with the occurrence of gallstones must take into account not only the analytical data summarized above, but, probably, also a previous finding according to which the bile lecithin from hamsters receiving a diet with 10% of the high linoleic acid margarine fat contained much more linoleic acid and correspondingly less oleic acid than did the bile lecithin from hamsters receiving a diet with 10% butter fat.

DokumentationCurrent literature. No. 4 by E. Ühlein (108-218).