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Biochemical Genetics (v.41, #1-2)
Genetic Variation in the Midcontinental Population of Sandhill Cranes, Grus canadensis by Jessica L. Petersen; Richard Bischof; Gary L. Krapu; Allen L. Szalanski (pp. 1-12).
Three subspecies of sandhill crane (Grus canadensis) are recognized in the Midcontinental population, the lesser (Grus c. canadensis), Canadian (G. c. rowani), and greater (G. c. tabida). Blood samples collected on the population's primary spring staging area in Nebraska, U.S.A., were used to resolve the genetic relationship among these subspecies. Phylogenetic analysis of 27 G. canadensis, by DNA sequencing of a 675 bp region of the mtDNA, supports the subspecies designations of G. c. canadensis and G. c. tabida. G. c. rowani individuals were intermediate with each of the other two subspecies. Genetic divergence ranged from 6.5 to 14.5% between G. c. canadensis and G. c. tabida, 0.5 to 6.6% within G. c. canadensis, and 0.1 to 6.0% within G. c. tabida. Sufficient DNA for analysis was obtained from shed feathers indicating a source of genetic material that does not require the capture or sacrifice of the birds. Other genetic markers and methods, including satellite telemetry, are required for obtaining detailed information on crane distributions as needed to establish effective management units for the MCP.
Keywords: Grus canadensis ; sandhill crane; genetic variation; Midcontinental population; mtDNA
Cloning, Expression, and Genomic Structure of a Novel Human Rap2 Interacting Gene (RPIP9) by Shu Wang; Zhen Zhang; Kang Ying; Jin-zhong Chen; Xian-fang Meng; Quan-Sheng Yang; Yi Xie; Yu-min Mao (pp. 13-25).
During a large-scale screen of a human fetal brain cDNA library, a full-length cDNA encoding a novel Rap2 interacting protein was isolated and sequenced. The cDNA is 3397 bp long and has a predicted open reading frame encoding a protein of 329 aa. The predicted protein shows high homology to mouse and human RPIP8, and has a RUN domain near its C-terminus. The gene was mapped to human chromosome 7q21–7q22 and has 9 exons and 8 introns. The expression pattern was also detected by cycle-limited reverse polymerase chain reaction (RT-PCR).
Keywords: Ras; GEF; RPIP9; RT-PCR; chromosome 7q21–7q22
Twelve Single Nucleotide Polymorphisms on Chromosome 19q13.2-13.3: Linkage Disequilibria and Associations with Basal Cell Carcinoma in Danish Psoriatic Patients by Jiaoyang Yin; Ulla Vogel; Lars Ulrik Gerdes; Marianne Dybdahl; Lars Bolund; Bjørn Andersen Nexø (pp. 27-37).
The genetic susceptibility to basal cell carcinoma (BCC) among Danish psoriatic patients was investigated in association studies with 12 single nucleotide polymorphisms on chromosome 19q13.2-3. The results show a significant association between BCC and the A-allele of a polymorphism in ERCCI exon4 (Odds ratio 12;95% Confidence Interval 1.17–124; p(χ2, two-side) = 0.019) and to a lesser extent with XPD exon6 (p = 0.06). This is in accordance with recent studies of a different group of BCC cases (Rockenbauer et al. (in press) Carcinogenesis; Yin et al. (manuscript submitted for publication). Cancer Epidemiol. Biomarkers Prev), which places two highly influential markers between these two genes. The analysis also confirmed that considerable linkage disequilibrium exists between SNPs both within genes and between genes in this region. The combined studies suggest that genetic variation in nucleotide excision repair is of importance for the development of BCC.
Keywords: linkage disequilibria; basal cell carcinoma; psoriasis; nucleotide polymorphism; epidemiology; basal cell carcinoma; DNA repair
High Mobility Group-Like Proteins of the Insect Plodia interpunctella by Vassiliki Aleporou-Marinou; Yianis Drosos; Yiannis Ninios; Barbara Agelopoulou; Theocharis Patargias (pp. 39-46).
Nuclei from Plodia interpunctella larvae contain four major proteins, which are extracted by 5% perchloric acid and 0.35 M NaCl. The proteins have been designated PL1, PL2, PL3, and PL4. The amino acid analyses of these proteins show that they have high proportions of acidic and basic amino acid residues, a property characteristic of the high mobility group (HMG) proteins isolated from vertebrate tissues. Immunological characterization of these proteins clearly shows that PL1, PL2, and PL4 are more closely related to HMG1 dipteran proteins, while PL3 is more closely related to HMGI dipteran proteins. The possible relatedness of these proteins to HMG proteins is discussed.
Keywords: HMG proteins; high mobility group-like proteins; polyacrylamide electrophoresis; western immunoblotting
Allozyme Variation and Population Differentiation of the Aconitum delavayi Complex (Ranunculaceae) in the Hengduan Mountains of China by Fumin Zhang; Hongzhi Kong; Song Ge (pp. 47-55).
The Aconitum delavayi complex is a group of four climbing species with trisect-leaves occurring in the Hengduan Mountains. The species of this complex are highly localized on very narrow regions with quite small population sizes. Because of rapid environmental changes recently in the Hengduan Mountains, this complex shows complicated morphological variability, which makes it difficult to delimit species. In the present study, 10 enzyme systems coding for 14 putative loci were employed to detect the interspecific and intraspecific genetic variation of the complex. In addition to low genetic diversity within all eight populations surveyed, the results indicate that A. episcopale is a distinct species because of high genetic identities among its three populations. Very low genetic divergence among populations of A. stapfianum and A. delavayi suggests that the two species should be treated as a single one.
Keywords: Aconitum delavayi complex; allozyme; taxonomy; Hengduan Mountains