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Biochemical Genetics (v.38, #3-4)

Identification and Characterization of Malaysian River Catfish, Mystus nemurus (C&V): RAPD and AFLP Analysis by L. K. Chong; S. G. Tan; K. Yusoff; S. S. Siraj (pp. 63-76).

This work represents the first application of the amplified fragment length polymorphism (AFLP) technique and the random amplified polymorphic DNA (RAPD) technique in the study of genetic variation within and among five geographical populations of M. nemurus. Four AFLP primer combinations and nine RAPD primers detected a total of 158 and 42 polymorphic markers, respectively. The results of AFLP and RAPD analysis provide similar conclusions as far as the population clustering analysis is concerned. The Sarawak population, which is located on Borneo Island, clustered by itself and was thus isolated from the rest of the populations located in Peninsular Malaysia. Both marker systems revealed high genetic variability within the Universiti Putra Malaysia (UPM) and Sarawak populations. Three subgroups each from the Kedah, Perak, and Sarawak populations were detected by AFLP but not by RAPD. Unique AFLP fingerprints were also observed in some unusual genotypes sampled in Sarawak. This indicates that AFLP may be a more efficient marker system than RAPD for identifying genotypes within populations.

Keywords: AFLP; RAPD; polymorphism; Mystus nemurus.

Biochemical Polymorphism in Yellow Catfish, Mystus nemurus (C&V), from Thailand by Sa-Nga Leesa-Nga; Siti Shapor Siraj; Siti Khalijah Daud; Panom K. Sodsuk; Soon Guan Tan; Srirat Sodsuk (pp. 77-86).

Yellow catfish, Mystus nemurus (Cuv. & Val.), is becoming one of the major freshwater species farmed by aquaculturists in Southeast Asia. It was of interest to examine levels of genetic subpopulation differentiation among samples of this species obtained from parts of its range, as well as to compare the genetics of wild and hatchery-bred fish. Horizontal starch gel electrophoresis and histochemical staining techniques were used to examine genetic variation within and among eight wild and one hatchery populations of M. nemurus from northern, northeastern, central and southern Thailand. Four tissues (heart, liver, kidney, and muscle) from individual specimens were used to analyze variations at 23 protein-coding loci. Fifteen of the 23 loci examined (65.22%), namely, ACP*, AAT-1*, EST-1*, EST-2*, GPI*, IDH-1*, IDH-2*, MDH-1*, MDH-2*, MDH-3*, ME*, PGM*, 6PGD*, SOD*, and HB*,were polymorphic at the 0.95 level. Observed heterozygosities ranged from 0.041 to 0.111, with an average of 0.068 ± 0.028. Genetic distances ranged from 0.005 to 0.164. The greatest genetic distance was found between the Chainat and the Suratthani populations (0.164), a level indicative of subspecific differentiation in M. nemurus from within Thailand.

Keywords: Mystus nemurus ; yellow catfish; genetic variation; electrophoresis; allozymes

New Genetic Variation in European Hares, Lepus granatensis and L. europaeus by Paulo C. Alves; Madalena Branco; Osório Matias; Nuno Ferrand (pp. 87-96).

Six genetic polymorphisms for the Iberian hare (Lepus granatensis) and four for the brown hare (L. europaeus) are newly described. The genetic variation of peptidases B (PEPB) and C (PEPC), hemoglobin CHKαCHK chain (HBA), hemopexin (HPX), vitamin D binding protein (GC), and properdin factor B (BF) was assessed by conventional electrophoresis and isoelectric focusing in carrier ampholytes and hybrid pH gradients. Six alleles were detected in PEPB, three in PEPC, four in HBA, six in GC, five in HPX, and six in BF. At least one allele was shared between species at all loci except HBA. The allelic overlap between the two species was medium to high in PEPB, GC, and HPX and small in PEPC and BF.

Keywords: iberian hare; brown hare; genetic polymorphism; peptidases; hemoglobin; α chain; plasma proteins

Heterologous Amplification of Microsatellite Loci from Mouse and Rat in Oryzomyine and Thomasomyine South American Rodents by C. André V. Lima-Rosa; Mara H. Hutz; Luiz F. B. Oliveira; Jaqueline Andrades-Miranda; Margarete S. Mattevi (pp. 97-107).

Eleven heterologous primers were investigated in 119 individuals of 11 species of rodents of the Oryzomyine and Thomasomyine groups. The animals were collected at four sites of the “Cerrado,” a dryland biome located on the Brazilian Plateau, all of them being karyotyped and taxonomically allocated according to the karyotype. Four of these primers, R47, R65, R75 (from Rattus), and ATP (from Mus) cross-amplified in at least one of these taxa, giving products of seven, nine, one, and three bands, respectively. These values are of the same order as others obtained when heterologous primers were amplified in other orders of mammals. Of the 20 products amplified in these two rodent groups by these four primers, only 7 of the bands were seen in a heteromorphic state (one individual presenting two bands), in two species (Rhipidomys aff. leucodactylus and Oryzomys megacephalus). The others occurred as monomorphic bands.

Keywords: oryzomyine; Thomasomyine; DNA microsatellite; heterologous amplification; rodent

Erratum (pp. 109-110).

Molecular Cloning and Tissue Expression Analysis of the β Subunit of Elongation Factor 1 in the Mouse by Qing Liu; Long Yu; Qiang Fu; Tieming Lang; Jianxuan Zhang; Daqin Mao; Shouyuan Zhao (pp. 111-117).

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