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Introduction to Photostability
A Practical Introduction to
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Tandem Mass Spectrometry of Biological Molecules
| Type | Course |
|---|---|
| Language | English |
| Date |
August 19, 2009
to August 20, 2009 |
| Venue |
Crowne Plaza Hotel - The Hamilton 1001 14th St NW Washington, DC 20005 US |
| Chemistry Specialties |
|
| Chemistry Techniques |
|
| Contact |
American Chemical Society 1155 Sixteenth Street, NW Washington, DC 20036 US (800) 227-5558 shortcourses@acs.org |
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Most mass spectra recorded from biological molecules are based on fragmentations of even-electron ions. However, there are no books dedicated to interpretation of even-electron-ion spectra. Traditional, well-established fragmentations mechanisms are pertinent only to odd-electron ions derived by electron ionization procedures. This new course is designed to fill this void.
Key Topics
* Generating gaseous ions and separating them according to mass.
* Isolating gaseous ions of interest.
* Fragmenting an isolated ion and generating a mass spectrum.
* Comparing strengths and limitations of different tandem mass spectrometric techniques.
* Interpreting tandem mass spectrometric product ion spectra of positive and negative ions.
* Obtaining quantitative data from your sample by tandem mass spectrometry.
* Deciding the best techniques for your sample analysis.
* Dealing with how to buy a tandem mass spectrometer suitable for solving your analytical problems.
* Details about facts your vendor might not tell you.
Session titles
• Basic concepts of Tandem Mass Spectrometry
• Instrumentation for Tandem Mass Spectrometry (tandem in space and time techniques)
• Isolation and fragmentation of gaseous ions (CID, SORI, ECD, ETD)
• Post-source decay (PSD), and linked scans techniques
• Fragmentation mechanisms of even-electron positive and negative Ions
• MS/MS of amino acids, peptides, carbohydrates, lipids, and small organic molecules
• Interfacing chromatographs with tandem mass spectrometers
• Quantification (SIM, MRM) of Biological, Pharmacological, and Forensic samples