Sample Preparation for the LC-MS/MS Analysis of Biofluids

Type	Training - Paid
Language	English
Date	December 3, 2010 from 09:00 am to 04:30 pm
Venue	Royal Institution of Great Britain 21 Albemarle Street London, W1S 4BS GB
Chemistry Specialties	analytical chemistry
Chemistry Techniques	separation science
Contact	Jackie Tan Separation Science Frederick House, Princes Court, Beam Heath Way Nantwich, CW5 6PQ GB 44 (0)208 398 3684 44 (0)208 711 3894 jackie.tan@sepscience.com
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Participants will learn about the principles and application of multidimensional SPE for highly selective sample clean-up. Properties and performance of tailor-made SPE packing materials (e.g., restricted access materials, molecularly imprinted polymers, mixed-mode materials, etc.), as well as high throughput and miniaturization of SPE-LC will be described in detail. Besides direct injection and on-line SPE of urine and plasma samples, the direct injection and in-line processing of whole blood will be addressed. Also discussed will be the pros and cons of dried blood spot (DBS) sampling and analysis, and a general introduction to the understanding and monitoring of ion suppression/matrix effects in bioanalytical LC-MS/MS will be given. A focus will be on the elimination of ion suppression by an adequate sample pretreatment and separation. In this context, Phase Optimized Liquid Chromatography (POPLC) will be described as a valuable tool. A broad spectrum of applications in the fields of bioanalysis such as therapeutic drug monitoring, biological monitoring, environmental– and clinical-chemical analysis will be presented. Topics covered in the course include: • Basic goals of sample cleanup • Solid-phase extraction • Automation strategies • SPE-LC • Tailor-made SPE sorbents • Instrumentation • Method development • SPE-LC-MS/MS • Complex biofluids • Whole blood processing • Application examples

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