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Capillary Electrophoresis of Biomolecules

Type Course
Language English
Date August 15, 2009 to
August 16, 2009
Venue Four Points By Sheraton
1201 K Street NW
Washington, DC 20005
US
Chemistry Specialties
  • other
Chemistry Techniques
  • other
Contact
American Chemical Society
1155 Sixteenth Street, NW
Washington, DC 20036
US
(800) 227-5558

shortcourses@acs.org
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by Daniel Fishman last modified 05-21-09 04:46 AM

Capillary electrophoresis is increasingly employed to replace labor intensive slab-gel methods. This two (2) day short course is designed for those new to CE although experienced users who wish to broaden their background will especially benefit. The course begins with an extensive review describing that factors that influence the electrophoretic migration of molecules in fluid solution. A phenomological approach is employed that enables the student to take home a thorough understanding of electrophoretic processes. The instructor uses examples ranging from the simple to the complex to explain the movement of ions in fluid solution under the influence of the applied field. Because of this, by the end of this two-day course, you will gain an in-depth understanding of electrophoretic events and methods development and troubleshooting will rapidly become an intuitive process. Key Topics * How to implement capillary electrophoresis to replace and/or complement slab-gel electrophoresis * The mechanisms of capillary electrophoretic separations from a phenomenological viewpoint * How to optimize the background electrolyte for problem separations * Where to locate the best sources of technical information * How to prepare samples that are optimized for capillary electrophoresis * Considerations for method transfer and validation

Who Should Attend? Scientists who are involved with separation and determination of biomolecules such as amino acids, proteins, peptides, and carbohydrates. Separations of DNA for many applications are covered as well. These applications include sequencing, restriction digests, short tandem repeats, plasmids. oligonucleotides and various modes of genetic analysis.

Speakers

Robert Weinberger is the President of CE Technologies, Inc., a contract research laboratory specializing in capillary electrophoresis.

Session titles

Introduction Electrophoretic migration of ions * Ohm's law * Historical perspective * Electro osmosis * Efficiency and resolution * Optimization of electrophoretic variables * Buffers * Commercial instrumentation Capillary Zone Electrophoresis of proteins and peptides * Mobility plots * Wall effects * Coatings and dynamic coatings * Secondary equilibrium and micellar electrokinetic separations * Developing a well controlled separation * Sample preparation and salt effects Capillary Isoelectric Focusing * Separation mechanism * Capillary and ampholyte selection * Sample preparation and injection * Mobilization schemes * Imaged detection * Selected applications Capillary Sieving Electrophoresis * Gels versus capillary separations * Introduction to polymer networks * Applications for DNA separations including sequencing * Separation of SDS-proteins * Optimization and validation Injection and detection * Hydrodynamic versus electrokinetic injection * Optimization of injection * Ultraviolet and laser induced fluorescence detection * Mass spectroscopy * Carbohydrates Troubleshooting Guide Stacking and Trace Enrichment * Ionic strength mediated stacking * pH mediated stacking * Field amplified stacking Microfabricated Systems Validation and Methods Transfer * ICH guidelines * Quantitative analysis * Analytical figures of merit * Documentation and training * Failure analysis * Case studies

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